First, never delete critical files under inetpub. Folders such as adminscripts, custerr, logs, history, temp have their usage. For example, IIS writes temporary files into temp folder.
Second, 401.3 means IIS cannot access the new web site content folder as permissions are not enough. If you did not delete wwwroot, you can check its security settings and compare to the new folder.
I suggest you start all over again from another clean install.
Regards,