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Last post 04-08-2009 8:28 PM by lextm. 3 replies.
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First, please roll back the changes you have made.
Second, try to see if you are facing one of the breaking changes here,
http://mvolo.com/blogs/serverside/archive/2007/12/08/IIS-7.0-Breaking-Changes-ASP.NET-2.0-applications-Integrated-mode.aspx
If you did not try Classic pipeline mode for the application pool, please have a try.
If this still failed, you may use failed request tracing to collect more information about which module raises 500.0.
http://learn.iis.net/page.aspx/266/troubleshooting-failed-requests-using-tracing-in-iis7/